Journal: Journal of cancer research and therapeutics

Article Title: PRDX3 promotes resistance to cisplatin in gastric cancer cells.

doi: 10.4103/jcrt.jcrt_970_22

Figure Lengend Snippet: Figure 2: PRDX3 is downregulated by siRNA in AGS and MKN‑74 cells. (a) Relative mRNA expression of PRDX3 in AGS after transfection with siNC and four siRNAs. (b) Relative mRNA expression of PRDX3 in MKN‑74 after transfection with siNC and four siRNAs. (c) Relative PRDX3 protein expression in AGS after transfection with siNC and siPRDX3. (d) Relative PRDX3 protein expression in MKN‑74 after transfection with siNC and siPRDX3. (e) Western blot bands of PRDX3 protein in AGS and MKN‑74 after transfection with siNC and siPRDX3

Article Snippet: PRDX3 interfering RNA (siPRDX3 group) and negative control (siNC group) were transfected according to the instructions of the siTran1.0 transfection kit (Origene), and the blank control group was not transfected.

Techniques: Expressing, Transfection, Western Blot

Journal: Journal of cancer research and therapeutics

Article Title: PRDX3 promotes resistance to cisplatin in gastric cancer cells.

doi: 10.4103/jcrt.jcrt_970_22

Figure Lengend Snippet: Figure 1: PRDX3 expression level in adjacent noncancer and gastric cancer tissue (original magnification, ×400). (a) Immunohistochemistry images of normal gastric tissue. (b) Immunohistochemistry images of moderately differentiated adenocarcinoma. (c) Immunohistochemistry images of poorly differentiated adenocarcinoma. (d) PRDX3 expression in gastric cancer tissue was significantly higher than in adjacent noncancer tissue. **P < 0.01

Article Snippet: PRDX3 interfering RNA (siPRDX3 group) and negative control (siNC group) were transfected according to the instructions of the siTran1.0 transfection kit (Origene), and the blank control group was not transfected.

Techniques: Expressing, Immunohistochemistry

Journal: Journal of cancer research and therapeutics

Article Title: PRDX3 promotes resistance to cisplatin in gastric cancer cells.

doi: 10.4103/jcrt.jcrt_970_22

Figure Lengend Snippet: Figure 3: IC50 values of each group after PRDX3 knockdown in AGS and MKN‑74 cells. (a) Control (CON) group of AGS. (b) siNC group of AGS. (c) siPRDX3 group of AGS. (d) CON group of MKN‑74. (e) siNC group of MKN‑74. (f) siPRDX3 group for MKN‑74. (g) Comparative histogram of IC50 in each group in AGS cells. (h) Comparative histogram of IC50 in each group in MKN‑74 cells. **P < 0.01

Article Snippet: PRDX3 interfering RNA (siPRDX3 group) and negative control (siNC group) were transfected according to the instructions of the siTran1.0 transfection kit (Origene), and the blank control group was not transfected.

Techniques: Knockdown, Control

Journal: Journal of cancer research and therapeutics

Article Title: PRDX3 promotes resistance to cisplatin in gastric cancer cells.

doi: 10.4103/jcrt.jcrt_970_22

Figure Lengend Snippet: Figure 5: Expression level of the related proteins in the apoptosis pathway in AGS and MKN‑74 cells transfected with siPRDX3 after cisplatin treatment. ***P < 0.001. (a) Western blot bands. (b) Relative expression of cleaved caspase‑3 protein in each group of AGS cells. (c) Relative expression of Bax protein in each group of AGS cells. (d) Relative expression of PRDX3 protein in each group of AGS cells. (e) Relative expression of cleaved caspase‑3 protein in each group of MKN‑74 cells. (f) Relative expression of Bax protein in each group of MKN‑74 cells. (g) Relative expression of PRDX3 protein in each group of MKN‑74 cells

Article Snippet: PRDX3 interfering RNA (siPRDX3 group) and negative control (siNC group) were transfected according to the instructions of the siTran1.0 transfection kit (Origene), and the blank control group was not transfected.

Techniques: Expressing, Transfection, Western Blot

Journal: Immune Network

Article Title: Peroxiredoxin-3 Is Involved in Bactericidal Activity through the Regulation of Mitochondrial Reactive Oxygen Species

doi: 10.4110/in.2016.16.6.373

Figure Lengend Snippet: Peroxiredoxin-3 (Prdx3) is implicated in the generation of mitochondrial reactive oxygen species. (A) HEK293T cells were fractionated and protein extracts were analyzed by western-blotting with antibodies for Prdx3, IκB-α for cytoplasmic marker or GRIM19 for mitochondrial marker. The western blotting shown is representative of three independent experiments. (B) HEK293T cells were fixed and immunostained with MitoTracker (green) and an anti-Prdx3 antibody (red), and counterstained with 4’,6-diamidino-2-phenylindole (blue). Data are representative of three independent experiments. (C) THP-1 cells were infected with lentivirus containing shRNA targeted to human Prdx3, or control lentivirus, according to the manufacture's protocol. Control THP-1 (Ctrl) and Prdx3-knockdown THP-1 (Prdx3 KD THP-1) cells were cultured in puromycin-containing medium (4 µg/mL) for 2 weeks to select stable clones. Immunoblotting with anti-Prdx3 or anti-GAPDH antibodies was performed to evaluate the knockdown efficacy. The western blotting shown is representative of three independent experiments. (D) Ctrl and Prdx3 KD THP-1 cells were cultured without or with 500 ng/mLlipopolysaccharide (LPS) for 60 min, stained with MitoSOX, and analyzed by immunofluorescence microscopy. Data are representative of three independent replicates. (E) Ctrl and Prdx3 KD THP-1 cells were cultured without or with 500 ng/mL LPS for 60 min, stained with MitoSOX, and analyzed by flow cytometry. Data are presented as the mean fluorescence intensity (M.F.I)±SEM from triplicate samples. * p<0.05, ** p<0.01.

Article Snippet: Lentiviruscontaining small hairpin RNA (shRNA) targeting human Prdx3 (sc-40833-V) and control shRNA lentivirus (sc-108080) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Western Blot, Marker, Infection, shRNA, Control, Knockdown, Cell Culture, Clone Assay, Staining, Immunofluorescence, Microscopy, Flow Cytometry, Fluorescence